Tuesday, February 1, 2011

Human Physiology: Cardiovascular Physiology

Study Guide – Cardiovascular Physiology

General Features of the Cardiovascular System

overview

- functions of the cardiovascular system

- maintenance of homeostasis

- amount of blood flow to each organ must be matched to the needs of the organ

- distribution of blood flow must be adjusted continuously and rapidly

- regulation of body temperature

- variation in blood flow to the skin

- increased temperature leads to increased peripheral blood flow

- transportation of hormones

- anatomical divisions of the cardiovascular system

- systemic circuit: left ventricle to body to right atrium

- pulmonary circuit: right ventricle to lungs to left atrium

- blood volume and distribution of blood between the circuits

- adult human volume

- 4-6 L total

- 70-75 mL blood / kg body weight

- measurement: Evan’s blue dye

- upon injection, binds to plasma proteins in the cardiovascular system

- known volume is injected, and after several minutes, blood sample is drawn and analyzed

- calculation:

- unequal blood distribution

- systemic: 80-85% of total

- pulmonary: 15-20% of total

autonomics in the nervous system

- parasympathetic

- M2 receptor

- effector: atrial muscle; cardiac nodal tissue (NOT present in ventricles)

- effect: contractile inhibition: slows heart rate, AP conduction velocity, and contractility

- mechanism: anti-β effects (↓ PKA via adenylate cyclase inhibition); ↑gK

- sympathetic

- α1 receptor

- effector: vascular smooth muscle (except coronary, cerebral, pulmonary circulation)

- effect: contraction: targeted vasoconstriction

- mechanism: PKC-induced ↑gCa and ↓gK, giving depolarization

- β1 receptor

- effector: cardiac muscle, nodal tissue

- effect: contractile: increases heart rate, AP conduction velocity, and contractility

- mechanism: PKA-induced ion channel and contractile protein phosphorylation

- β2 receptor

- effector: variable (sometimes present in heart, peripheral vasculature)

- effect: relaxation

- mechanism: PKA activation of Ca2+-dependent gK, giving hyperpolarization

basic anatomy of the systemic circulation

- vascular bed: set of blood vessels supplying blood to a given organ or type of tissue

- organization and order of flow: high to low pressure

- arterial tree: aorta, artery, arteriole, terminal arteriole

- capillary bed

- venous return: venule, vein, vena cava

- structures

- smooth muscle: higher content in high pressure vessels

- elastic tissue: higher content in high pressure vessels

- endothelial cells: line all vessels, including capillaries

- exchange: independent controls of vasoconstriction and vasodilation

- arterioles: regulated by sympathetic innervation

- precapillary sphincters: regulated by O2 tension, CO2 tension

- cross-sectional area

- varies immensely over different levels of circulation

- much larger in capillary beds

- larger area for exchange

- greater time for exchange (increased cross-sectional area means decreased velocity)

- systemic vascular beds: arrangement in parallel

- physiological advantages of parallel arrangement

- blood composition identical in each bed

- arterial pressure identical in each bed

- independent regulation of flow to each bed

- low resistance to flow vs. arrangement in series

- exceptions: arrangement in series

- portal circulations: specialized for point-point delivery of substances (hormones, nutrients)

- glomerular capillary beds: specialized for filtration of blood, reabsorption of salts and water

concepts and terms

- pressure

- pressure: form of energy driving cardiovascular blood flow

- sphygmomanometer: device commonly used to measure blood pressure in humans

- measurement of arterial blood pressure

- arterial blood pressure fluctuates in time, so multiple terms must be used

- systolic pressure: highest pressure achieved during the cardiac cycle (contraction phase)

- diastolic pressure: lowest pressure achieved during the cardiac cycle (relaxation phase)

- pulse pressure: arithmetic difference between systolic, diastolic pressure

- mean arterial blood pressure (MABP): average pressure during an entire cardiac cycle

- can be calculated by a measurement of area under the curve

- can be approximated as 1/3 of the pulse pressure, summed to diastolic pressure

- typical values

- males: 120/80 (systolic/diastolic)

- females: 110/70

- flow

- blood flow (Q): amount of blood flowing through a circuit per unit time

- equation:

- Q: blood flow

- ΔP: pressure gradient driving flow

- R: resistance to flow

- continuity of flow

- amount of blood flowing through any unique cross-sectional areas are identical

- deviations from continuity indicate disease, acute injury

- velocity of flow

- measurement: flux past a point in a circuit (cm/s)

- varies with cross-sectional area of the flow

- large arteries: 30-50 cm/sec

- capillaries: 0.1 cm/sec

- large veins: 15-20 cm/sec

Physiological Properties of Heart Muscle

automaticity

- automaticity: ability of the heart to spontaneously generate AP necessary for each beat

- pacemaker cells: automatic cells; drive pace of heart beat

- cardiac innervation: autonomic

- heart is not controlled via nerve-induced action potentials, as in α-motor neurons

- autonomic innervation regulates automaticity, force of muscle contraction

conduction or propagation of action potentials

- syncytium: collective; functional organization of heart

- mechanisms of propagation identical to those in unmyleinated neurons, skeletal muscle fibers

- APs pass readily from cell to cell, utilizing numerous gap junctions

- intercalated disc: specialized gap junction permitting local currents to flow from cell to cell

- features and consequences of conduction in the heart

- action potentials from a single cell eventually propagate throughout the entire heart

- all cells undergo excitation, contract during each beat

- not possible to vary force of heart beat by recruitment

- other mechanisms must be utilized

- stimulating electrodes of pacemakers can be placed on any part of the ventricles

- propagation velocity of action potentials varies greatly in different regions of the heart

- nodal tissue: as slow as 0.05 m/s

- ventricular conducting tissue: as fast as 5.0 m/s

contractility of myocardium

- similarities to skeletal muscle

- mechanoenzyme: actin-activated myosin ATPase

- regulation: Ca2+ release from the sarcoplasmic reticulum, binding to troponin C

- tension: attachment and cycling of myosin cross-bridges between thick, thin filaments

- differences from skeletal muscle

- no mechanical coupling between sarcolemma, sarcoplasmic reticulum (CICR still present)

- increased strength is not gained by motor unit recruitment

- Ca2+ entry into cell, Ca2+ release from the SR, Ca2+ SR reuptake are all regulated

- modulation of contractility typically involves regulation (phosphorylation) of these enzymes

- rates of chemical transitions within cross bridge interaction cycle subject to regulation

- contractility

- contractility: ability of the myocardium to develop tension and to shorten

- involves contractile force, rate of force development, and velocity of shortening

distensibility of myocardium

- distensibility: ability to be lengthened or stretched when a force is applied

- factors contributing to resting distensibility

- titin: protein that spans gap between Z line, ends of thick filaments, and gives passive stretch

- slowly-cycling cross bridges: at rest, some cross bridges still bind actin, and give force when stretched

- stretch influence on subsequent contractions

- number of cross bridges interacting with actin is greater at longer lengths

- amount of Ca2+ released from SR during a sarcolemmal action potential increases

- less physical distance between actin, myosin due to volumetric considerations

- thus length of vetricular cells at onset of each contraction affects force of contraction

refractoriness of myocardium

- refractory: unable to respond to an applied stimulus

- refractoriness in the heart

- begins with depolarization (upstroke), ends when membrane potential has repolarized to 50-90% of resting

- time: typically lasts several hundred milliseconds

- consequences

- refractory period lasts into myocardial relaxation phase, allowing relaxation between beats

- permits filling of the ventricles with blood prior to contraction, allowing more efficient pumping

Origin and Conduction of Cardiac Excitation

- anatomy: structures of cardiac conduction

- atria

- sinoatrial (SA) node

- interatrial and internodal tracts

- atrial muscle

- atrioventricular (AV) node

- ventricles

- Bundle of His

- right and left bundle branches

- terminal arborization (Purkinje tissue)

- ventricular muscle

action potential generation and conduction in normal heart

- conduction

- atrial contraction

- pacemaker: site of origin of the cardiac impulse (generally the SA node)

- conduction: originates in the SA node, spreads to AV node and atrial muscle via interatrial pathways

- contraction: virtually synchronous due to short distances of conduction

- AV node

- conduction: propagates through internodal tracts at a highly reduced velocity

- primarily responsible for delay between atrial, ventricular contractions

- ventricular contraction

- conduction: bundle of His through bundle branches, Purkinje system into terminal arborizations and muscle

- contraction: virtually synchronous (range <>

- conduction velocities

- SA node: 0.05 m/s

- internodal tracts: 1.0 m/s

- atrial muscle: 0.3 m/s

- AV node: 0.05 m/s

- His, Purkinje: 3.0 to 5.0 m/s

- ventricular muscle: 0.3 m/s

determinants of conduction velocity

- cell diameter: conduction velocity varies directly with diameter

- His-Purkinje cells: largest diameter cells in the heart, fastest conduction velocity

- mechanism: increased volume of charge carriers lowers axonal Ri

- factors in conduction velocity

- rate of depolarization during upstroke, depolarization phase of AP

- slow conduction: small upstroke velocities (5 V/s in nodal cells)

- fast conduction: large upstroke velocities (500 V/s in Purkinje cells)

- amplitude of AP overshoot (potential reversal)

- slow conduction: virtually no overshoot

- fast conduction: large overshoot (approaching +35 mV)

automaticity

- sinoatrial node: normal site of the cardiac pacemaker

- pacemaker current: analogous to K+ channels in muscle conduction, but with opposite direction

- designation: If (“funny”)

- activation: only activated at very negative potentials

- deactivation: self limiting; turns off as potential increases

- aspects of membrane potential in a cardiac nodal cell

- diastolic depolarization: slow, progressive depolarization in membrane potential

- occurs during diastole (between contractions) in cells exhibiting automaticity

- other names: phase 4 depolarization, pacemaker potential

- threshold potential: potential at which an action potential is initiated 50% of the time

- action potential: transient, propagating change in membrane potential

- results from regenerative increase in inward currents, subsequent activation of outward currents

- slow: APs in nodal cells depolarize slowly

- maximum diastolic potential: most negative membrane potential during diastole (follows end of AP)

- ion channels in a nodal action potential

- diastolic depolarization: inward Na+ (If)

- action potential: inward Ca2+

- repolarization: outward K+

- mechanisms for changing the automaticity of pacemaker cells

- biochemical methods

- alter rate of diastolic depolarization

- increased diastolic depolarization: increased APs

- decreased diastolic depolarization: decreased APs

- examples: caffeine, emotional stress, exercise

- alter threshold potential

- depolarization of threshold potential: decreased APs

- hyperpolarization of threshold potential: increased APs

- examples: certain antiarrhythmic drugs (INa, ICa blockers)

- alter maximum diastolic potential (MDP)

- depolarization of MDP: increased APs

- hyperpolarization of MDP: decreased APs

- physiological methods of decreasing heart rate

- block If (theoretical)

- increase parasympathetic output (M2 receptor agonists)

- decrease sympathetic output (β-blockers)

localization of automaticity

- describing automatic cardiac cells

- true pacemaker: cells in normal hearts that generate the AP leading to the heart beat (usually SA node)

- latent pacemaker: cardiac cells with the capability of automaticity, other than true pacemaker

- normally do not spontaneously generate AP

- cells with greatest degree of automaticity reach threshold first

- other automatic cells are preemptively excited by propagating APs generated by the true pacemaker

- SA node generally has greatest automaticity, dominating other automatic cells

- ectopic pacemaker: automatic cell outside SA node that generates an AP leading to one or more beats

- includes latent pacemakers that have become true pacemakers

- numerous physiological, pathological processes can cause this to occur

- inherent rates of discharge of automatic cells

- in the absence of extrinsic neural, chemical influences:

- SA node: 120 /min

- internodal tract: 80-100 /min

- AV node: 80-100 /min

- His-Purkinje system: 30-50 /min

- greater numbers of If channels in more automatic cells

- mechanisms for shifting the site of automaticity to ectopic regions

- selectively suppress the automaticity of the SA node

- allows the next most automatic cells (usually AV node) to become the (ectopic) pacemaker

- occurs with aging

- selectively enhance the automaticity of latent pacemaker cells

- cells achieve threshold before SA node, thus becoming the (ectopic) pacemaker

- block conduction between SA node, lower heart (e.g. block the SA node)

- SA node will continue to be the atrial pacemaker

- Purkinje cells, in absence of SA control, become ventricular pacemakers

- this causes asynchrony between contraction of the atria, ventricles

- automaticity vs. conduction velocity: two different characteristics of membrane potential

- automaticity: depends on rate of diastolic depolarization

- conduction velocity: depends on cell diameter, upstroke velocity, overshoot

Ionic Currents Underlying Cardiac Membrane Potentials

description of cardiac action potentials

- diastolic depolarization

- not present in atrial, ventricular myocytes

- in all other tissues, automaticity declines with distance from SA node in normal conduction

- characteristics of specific action potentials

- ventricular myocyte action potential

- fast action potential: high upstroke velocity

- no diastolic depolarization (If): steady potential at rest

- refractoriness absolute: persists for most of the action potential

relative: during last stages of repolarization

- Purkinje action potential

- hybrid of ventricular, nodal action potentials

- undergoes diastolic depolarization, but at a lower rate than nodal cells

- bundle branches: longest action potentials

ionic basis for cardiac Em

- phase 0: upstroke, overshoot of the action potential

- atrial, internodal tract, ventricular, and Purkinje cells

- mechanism: fast Na+ channels

- upon triggering, Na+ channels rapidly open to allow influx of Na+ ions, depolarization

- with depolarization, Na+ channels rapidly inactivate, usually closing before ENa is reached

- K+ channels are used to reestablish polarity

- speed: rapid

- velocity of depolarization: 150 to 500 V/sec

- conduction velocity: 0.3 to 5.0 m/s

- variability in upstroke and conduction: Na+ channel inactivation

- as membrane potential becomes less negative, more Na+ channels are inactivated

- at a resting potential of -100 mV, almost all channels are available (closed, but not inactive)

- at a resting potential of -60 mV, almost all channels are unavailable (inactive)

- action potential overshoot (and thus conduction velocity) depends on initial number of available channels

- initial resting depolarization can therefore significantly slow conduction velocity

- tetrodotoxin: selectively blocks Na+ channels, preventing depolarization

- SA and AV nodal cells

- mechanism: slow L-type Ca2+ channels (DHP receptors)

- channels conduct both Ca2+ and Na+, but predominantly Ca2+

- AP triggering causes influx of Ca2+, while depolarization causes inactivation of channels

- K+ channels are used to reestablish polarity

- speed: slow

- both activation and inactivation of Ca2+ channels is slow, so overshoot and conduction is decreased

- velocity of depolarization: 5-10 V/s; overshoot <>

- note: slow L-type Ca2+ channels are found in all non-nodal cells as well

- Ca2+ current contributes to phase 2 (plateau phase)

- slow incoming Ca2+ current maintains depolarized state

- subsequent inactivation allows repolarizing channels to take over, repolarize myocyte

- L-type Ca2+ can also allow contraction in partially depolarized non-nodal cells

- at -60 mV, Na+ channels are almost all inactive

- cells can still undergo a slow action potential utilizing L-type Ca2+ channels

- in this case, contraction is slow and resembles that of nodal tissue

- phase 1: initial rapid repolarization

- mechanism

- inactivation of the fast Na+ current

- activation of the transient outward K+ current (Ito)

- localization

- Purkinje cells: prominent

- SA, AV cells: absent

- phase 2: plateau of the action potential (distinguishing characteristic of cardiac AP)

- mechanisms

- L-type Ca2+ current: flows for 200 msec or longer, maintaining positive membrane potential

- IK: gradually increasing conductance to K+ during the plateau

- during the plateau, L-type ICa dominates

- phase 3: repolarization to resting potential (or maximum diastolic potential)

- three separate mechanisms

- L-type Ca2+ current: eventually slows due to inactivation

- IK: continued increased membrane permeability to K+

- Na+/K+ ATPase: electrogenic effects

- repolarization brings potential back to its diastolic level

- phase 4: resting potential / diastolic depolarization

- non-automatic cells

- effect: steady diastolic resting potential (about -90 mV)

- mechanism: high permeability to K+ via K1 channel

- K1 channel: only open at very negative Em, maintaining polarity

- NOT the same channel as that mediating repolarization

- automatic cells

- effect: progressive diastolic (phase 4) depolarization, resulting in threshold and spontaneous AP trigger

- three mechanisms

- IK channels: time- and voltage-dependent closure, allowing Em to subsequently depolarize

- If channels: specialized channels that activate near maximum diastolic potential

- similar to potassium channels that cause repolarization, but work in the opposite direction

- direct cause of membrane depolarization

- T-type Ca2+ channels: activate near maximum diastolic potential, allowing inward Ca2+ current

- numerous mechanisms may be a consequence of the number of types of cells that can act as pacemakers

TABLE: Summary of Cardiac Action Potentials

phase

nodal cell

ventricular myocyte

Purkinje cell

phase 4:

diastole

↑ If (Na+)

↑ gCa (T-type ICa)

↓ gK (IK)

high gK (IK1)

↑ If (Na+)

↑ gCa (T-type ICa)

↓ gK (IK)

phase 0:

upstroke

↑ gCa (L-type ICa)

↑ gNa (TTX-sensitive)

↑ gCa (L-type ICa)

phase 1:

initial repolarization

---

↑ gK (Ito)

↑ gK (Ito)

phase 2:

plateau

---

↑ gCa (L-type ICa)

↓ gNa (TTX-sensitive)

↑ gK (IK)

↑ gCa (L-type ICa)

↓ gNa (TTX-sensitive)

↑ gK (IK)

phase 3:

repolarization

↑↑ g­K (IK)

↑↑ gK (IK)

↑↑ gK (IK)

Electrocardiography

theory of electrocardiography

- electrocardiogram: record of electrical changes within the heart as recorded from body surface

- basis of the EKG

- arises from potential differences between regions of the heart during spread of activation

- during activation of the heart, potentials change in a predictable and reproducible pattern

- uneven distribution of cardiac electrical activity can be detected via skin electrodes as potential difference

- model

- potential = (recording – reference)

- depolarization wave movement towards a recording electrode, away from a reference electrode gives (+) ECG

- electrocardiography as a diagnostic tool

- heart rate, rhythm

- pattern of conduction

- site of origin of the pacemaker

- sequence and time required for conduction through the heart

- diseases

- arrythmias

- conduction abnormalities

- hypertrophy (not always bad)

- ischemia

- necrosis

electrocardiography leads

- EKG leads

- lead: any pair of points on the body surface where electrodes are placed to record the EKG

- reference (–) electrode: serves as the reference for potential difference

- recording (+) electrode: number that is shown relative to the reference

- PD = (+) – (–): potential difference (PD) = recording (+) – reference (–)

- interference

- skin potentials are much less (maximum: 1-2 mV) than what would be measured directly on the heart surface

- EKG is thus susceptible to interference from other electrical fields

- external: shield EKG cables and recording devices

- muscular: hold still to maintain 0 mV baseline

- standard limb leads: depolarization movement in the frontal plane (Einthoven’s triangle)

- lead I: measures left arm (+) from right arm (–)

- lead II: measures left leg (+) from right arm (–) +60º

- lead III: measures left leg (+) from left arm (–) +120º

- augmented limb leads: depolarization movement in the frontal plane (supplemental)

- aVF: measures feet from center +90º

- aVR: measures right arm from center -150º

- aVL: measures left arm from center -30º

- reference: average of the other two limb electrodes (e.g. for aVF, reference is shorted left and right arms)

- precordial leads: depolarization movement in the transverse plane (anterior, posterior heart surfaces)

- V1: between ribs 4 and 5, right side of sternum (right ventricle)

- V2: between ribs 4 and 5, left side of sternum (right ventricle, septum)

- V3: curve inferiorly and laterally towards the side of the chest (septum)

- V4: curve inferiorly and laterally towards the side of the chest (septum, left ventricle)

- V5: curve inferiorly and laterally towards the side of the chest (left ventricle)

- V6: side of the chest (left ventricle)

- reference: sum of all three limb leads (thus reference in middle of back)

- does not visualize posterior aspect of the heart very well

- if a problem is suspected there, leads will be placed on the back instead

vector representations of the spread of activation

- patterns

- moving depolarization wave

- towards lead: positive amplitude

- parallel to lead: maximum amplitude

- perpendicular to lead: zero amplitude

- away from lead: negative amplitude

- moving repolarization wave

- towards lead: negative amplitude

- away from lead: positive amplitude

- tissue mass

- increasing: increased amplitude

- decreasing: decreased amplitude

- resting tissue

- uniform depolarization: zero amplitude

- resting potential: zero amplitude

- vector basis of measurement

- formally, EKGs measure surface charge densities

- informally, most think of it as a current wave (spread of activation, or local circuit current)

- ventricles: depolarization from right to left

- myocardium: depolarization from endocardium to epicardium

repolarization from epicardium to endocardium

(i.e. “there and back”)

- mean QRS vector: spatial sum of all instantaneous vectors

- instantaneous vector: direction of charge density movement at a particular moment

- mean QRS vector in young adults is approximately +60º (60º below horizontal)

- conduction velocity and wave magnitude: inverse proportionality

- faster conduction: smaller wave

- slower conduction: larger wave

clinical EKG recording

- lead I electrocardiogram

- idealized lead I electrocardiogram

- P wave: atrial depolarization (positive)

- QRS complex: ventricular depolarization

- Q wave: septal depolarization (negative; may be absent)

- R wave: primary ventricular depolarization (positive)

- S wave: last part of ventricular depolarization (negative)

- T wave: ventricular repolarization (positive)

- waves

- Q wave: septal depolarization

- left bundle branch has branches that course back into, supply septum

- sum of these vectors makes the negative Q wave (not always present)

- R wave: ventricular depolarization (dominated by left ventricle)

- conduction through walls: endocardial à epicardial

- left ventricle is much larger, as is apex

- thus leftward conduction in left ventricle dominates R wave

- S wave: last component of ventricular depolarization (moving away from lead)

- T wave: ventricular repolarization

- endocardium: low number of K+ channels, and thus longer AP

- epicardium: high number of K+ channels, and thus shorter AP

- repolarization of left ventricle moves rightward, causing an upright T wave

- intervals

- P-R interval: corresponds to conduction time from SA node through AV node

- S-T segment: plateau of ventricular action potential; isopotential

- QRS complex: onset of ventricular depolarization

- Q-T interval: period of ventricular depolarization and repolarization

- long QT syndrome: caused by poor ventricular repolarization

- can be due to defective or blocked K+ channels (or high interstitial K+)

- can be due to defective or blocked Na+ channels

- nomenclature: other leads

- theory

- in all leads, Q, R, and S waves are assigned based on polarity and sequence

- in leads other than lead I, QRS waves do not necessarily correspond to identical electrical events

- naming

- Q wave: a negative wave preceding an R wave (sometimes absent)

- R wave: first positive wave in the QRS complex reporting ventricular depolarization

- lead I: reports ventricular depolarization

- V1: reports septal depolarization, which generally moves towards V1

- S wave: negative wave following an R wave

- lead I: final stages of ventricular depolarization

- V1: main phase of ventricular depolarization (moves to the left, away from V1)

reading an ECG

- protocol

- scan the ECG for technical adequacy (noise, signal size)

- measure heart rate, intervals, amplitudes, axis

- identify the rhythm (sinus, supraventricular, ventricular)

- describe abnormalities

- interpret the ECG, listing each abnormality

- compare with previous ECG, if available

- simple estimation of heart rate

- ECG paper

- minor divisions: 40 ms

- major divisions: 200 ms

- estimation

- QRS complex every major division: 300 /min

- QRS complex every two major divisions: 150 /min

- QRS complex every three major divisions: 100 /min

- QRS complex every four major divisions: 75 /min

- QRS complex every five major divisions: 60 /min

- QRS complex every six major divisions: 50 /min

- calculation of the QRS axis

- QRS axis: calculated variable that estimates net direction of depolarization of ventricles

- summation of all vectors generated during ventricular depolarization

- left ventricle is generally the largest tissue mass in normal hearts, so QRS axis usually points in that way

- calculation

- select two leads (typically I and aVF, since their horizontal/vertical direction is easiest for calculation)

- from isopotential, measure amplitudes of Q, R, and S waves

- subtract largest of Q or S wave from R wave

- plot vectors away from center

- direction: based on direction of the lead

- amplitude: based on calculation

- draw lines perpendicular to the end of each vector

- QRS axis: vector from center to the point where perpendicular lines intersect

- normal values: -20º to +110º

- less than -20º left-shifted pregnant, overweight: heart oriented more horizontally

LV hypertrophy: greater depolarization towards the left

- greater than +110º right-shifted thin: heart oriented more vertically

RV hypertrophy: greater depolarization towards the right

- note: angles are relative to lead I, with a positive value corresponding to the direction of right shifting

normal and abnormal 12-lead ECGs

- normal

- sinus rhythm

- rhythm of the heart driven by the sinoatrial node

- marked as regular appearance of a P wave at a constant interval before each QRS complex

- normal

- regular sinus rhythm of 60-100 bpm

- no evidence of pathological processes

- abnormal

- tachycardia: heart rate greater than 100 bpm

- bradycardia: heart rate less than 50 bpm

- atrial fibrillation

- arrhythmia with asynchronous APs arising from multiple ectopic foci in the atria

- seen as irregular baseline, absence of P waves, irregular ventricular rhythm due to irregular AV conduction

- ventricular rhythm

- rhythm of the heart beat driven by an ectopic pacemaker of ventricular origin

- seen as dissociation of P-waves and QRS complexes, and widened QRS complexes

- normal QRS width: less than 120 ms (3 boxes)

- ventricular fibrillation

- arrhythmia with asynchronous APs arising from multiple ectopic foci in the ventricles

- seen as continuous series of up/down deflections of variable amplitude and duration

- left ventricular hypertrophy

- enlargement of the left ventricle, either physiological (athlete) or pathological (hypertensive)

- seen as abnormally large QRS complexes in right or left ventricular leads

- left bundle branch block (LBBB)

- slowed conduction into the left ventricle

- seen as prolonged QRS complex, large R’ waves in left-side leads (I, V5, V6)

- R’ wave: second positive deflection (rabbit ear) caused by sequential ventricular depolarization; can be subtle

- right bundle branch block (RBBB)

- slowed conduction into the right ventricle

- seen as prolonged QRS complex, large R’ waves in right side leads (V1, V2) and/or broad left side S waves

The Heart as a Pump

cardiac valves

- atrioventricular valves: connected to papillary muscles by chordae tendinae

- tricuspid valve: divides right ventricle from right atrium

- bicuspid valve (mitral): divides left ventricle from left atrium

- semilunar valves: each has three cusps

- pulmonic valve: divides pulmonary artery from right ventricle

- aortic valve: divides aorta from left ventricle

- other structures

- sinuses of Valsava: bulges or spaces in arterial walls directly behind valve cusps

- ostia of coronary arteries: within sinus of Valsava in the aorta

- memory device: “ten pin bowling alley” (tricuspid, pulmonic, bicuspid, aortic)

- function: open or close depending on pressure gradient

- valves

- AV valves: close when ventricular pressure is greater than atrial pressure (supported by chordae tendinae)

- semilunar valves: close when arterial pressure is higher than ventricular pressure

- closure

- approximation (coaption): closure of the valve cusps

- insufficiency: failure of valves to fully close

cardiac cycle

- cycle

- ventricular systole: marked by closure of mitral valve, onset of isovolumic contraction

- isovolumetric contraction: ventricle builds tension/pressure, but does not exceed arterial pressure

- ventricular ejection

- rapid ejection: pressure rises to peak, volume drops rapidly

- reduced ejection: pressure falls from peak, volume drops slowly

- ventricular diastole: marked by closure of aortic valve

- isovolumetric relaxation: ventricle loses tension/pressure, aortic valve closes; dichrotic notch

- ventricular filling

- rapid filling: mitral valve reopens, blood rapidly enters ventricles due to atrial buildup (75-80% of filling)

- reduced filling: blood enters more slowly (15-20% of filling)

- atrial systole: contraction causing remaining filling of ventricular volume

- concepts

- dichrotic notch: transient increase in aortic pressure caused by backflow, rebound of blood off valve

- blood pressure

- diastolic: pressure at which aortic valve opens (between isovolumetric, rapid ejection)

- systolic: marked by peak of atrial pressure (between rapid, reduced ejection)

- volume

- end-systolic volume: residual ventricular volume (during isovolumetric relaxation)

- end-diastolic volume: peak of ventricular volume (during isovolumetric contraction)

- directly determines length of individual muscle fibers

- indirectly determines force muscle fibers will develop during subsequent contraction

- filling

- increasing heart rate causes proportional decrease in length of diastole, NOT in filling volume

- rapid filling early in diastole allows filling to remain steady even with increasing heart rate

- only beyond ~180 bpm does rapid filling phase decrease sufficiently to reduce filling volume

- during filling, ventricular pressures are generally less than pressures in great vessels

determinants of ventricular filling

- rate of venous return: greater return à greater filling (aided by ventricular relaxation)

- filling time: greater diastole à greater end-volume

- ventricular distensibility: greater distensibility (compliance) à greater end-volume

- stiff ventricles (e.g. hypertrophy) impairs filling, requiring greater venous pressure

- as ventricular distensibility decreases, atrial contraction becomes a more important contributor

atrial function

- reservoirs: store blood during period that AV valves are closed

- ventricular filling: increasingly important with:

- faster heart rates

- vascular stenosis

- hypertrophied ventricles (decreased distensibility)

- AV valve closure: aid by creating eddy currents that move cusps closer to center of AV orifice

- changes in left atrial pressure during cardiac cycle

- a wave: increasing pressure due to atrial systole

- c wave: transient increase in pressure during isovolumetric contraction (bulging AV valves)

- x descent: results from early ventricular ejection

- during ejection, base of ventricle moves down toward apex

- AV valve, atrium pulled downward, enlarging atrium and reducing pressure

- v wave: increasing pressure due to accumulation of blood within the atrium

- y descent: results from rapid ventricular filling, suction from ventricular relaxation

- left atrial pressure

- contraction: a wave

- ventricular filling/release: c wave, x descent

- atrial filling/release: v wave, y descent

characteristics of ejection

- stroke volume: amount of blood ejected into aorta during a beat

- average: 60-70 mL (60-70% of blood contained within ventricle)

- determinants: primarily force, velocity of ventricular muscle contraction

- end-systolic volume: residual volume contained within ventricle

- ejection fraction: measure of cardiac performance

- corrects for effect of body size on stroke volume

- formula:

- EDV: end-diastolic volume of the ventricle (maximum volume)

- ESV: end-systolic volume of the ventricle (minimum volume)

- SV: stroke volume (difference)

Regulation of Cardiac Function

key concepts

- preload: extent of diastolic filling (i.e. pre-loading of the ventricle)

- generally, as preload increases, stroke volume increases

- measured as EDV or EDP

- afterload: load (or pressure) against which the heart must work

- generally, as afterload increases, stroke volume decreases

- indicators of afterload

- mean arterial blood pressure

- ventricular wall stress (best indicator)

- regulation

- targets

- heart rate (beats / min)

- stroke volume (mL ejected / beat)

- cardiac output (CO): product of heart rate and stroke volume

- mechanisms

- intrinsic: exist within the heart, and do not involve neural control or circulation

- neural: nerve inputs to the heart

- types: parasympathetic, sympathetic

- targets: heart rate, force, and velocity of muscle contraction

- chemical: naturally-circulating or pharmacologic agents that bind receptors, interact with ion pumps

- heteromeric autoregulation

- changes in muscle length (ventricular volume) alter force of muscular contraction (ventricular pressure)

- mechanism: Frank-Starling mechanism

the Frank-Starling mechanism

- experiment

- design

- canine heart-lung preparation isolated from chemical, neural effects

- effects on cardiac performance studied with varying factors

- findings

- increased ventricular filling

- led to increased contractile force, stroke volume

- extra blood quickly pumped out in subsequent beats

- increased aortic pressure

- less blood pumped into the aorta during the first few transitional beats

- led to increased residual volume of the ventricles, stretched ventricular myocytes

- led to increased contractile force, returning stroke volume to normal

- cardiac performance

- ventricular function vs. chamber dimensions

- y: stroke volume, cardiac output, or stroke work

- x: venous pressure, atrial pressure, or ventricular end-diastolic pressure (VEDP)

- VEDP / VEDV: inputs

- preload: amount of blood and pressure present within the ventricles at the end of diastole

- afterload: pressure against which the heart works

- systemic arterial pressure: good indicator of afterload

- wall stress: best indicator of afterload

- wall stress as the best indicator of afterload

- LaPlace’s Law for spherical wall stress:

- P: ventricular pressure

- r: chamber radius

- t: wall thickness

- situations where mean arterial blood pressure (MABP) is a poor indicator

- aortic stenosis

- problem: narrowing of the aortic valve opening

- left ventricle must generate blood pressure far in excess of systemic pressure

- dilated cardiomyopathies

- problem: chamber radius is increased, wall thickness decreased relative to normal

- wall stress substantially greater than normal to maintain adequate arterial blood pressure

the basis for the Frank Starling relationship

- alteration in thick/thin filament overlap (minor)

- end-diastolic sarcomere lengths range from 1.8 to 2.3 μm

- below 1.8 μm, there is virtually no tension

- within plateau of length-tension relationship, substantial decreases in tension

- implication: most of variation must involve other mechanisms

- L-type channel-dependent release of Ca2+

- amount of Ca2+ released into myoplasm during EC coupling depends on length

- increased length (distension) leads to increased Ca2+ release, possibly due to:

- increased L-type current, Ca2+ entry into the cell

- increased sensitivity of the SR to the trigger Ca2+ entering the cell

- implication: increased stretch leads to increased Ca2+ and contractility

- alteration in probability of cross bridge binding (most important)

- constant volume system: in striated muscles, as cylinder length increases, diameter decreases

- increased distension leads to smaller distance between actin and myosin, increased rate constant for binding

- implication: increased stretch leads to greater probability of cross bridge formation

implications of the Frank Starling relationship

- systemic venous blood pressure is kept from rising to high levels

- output of the two ventricles is self-correcting

- stroke volume is normal in individuals with chronic hypertension

- work: work = P · ΔV

= mean arterial blood pressure · stroke volume

= MABP · SV

- acute hypertension

- under normal circumstances, MABP is well regulated and relatively constant

- with sudden hypertension, MABP increases, and work is initially constant, so SV decreases

- decreased SV à increased ESV à increased EDV à increased work capacity of the heart

- end result: increased work, increased MABP, normal stroke volume (metabolic burden)

- problem: hypertrophy can occur over time, ultimately leading to heart failure

Neural Regulatory Mechanisms

anatomy of autonomic innervation

- parasympathetic

- preganglionic

- cell bodies: medulla

- axons: project to peripheral ganglia via the vagus (X) verve

- neurotransmitter: acetylcholine

- postganglionic

- cell bodies: peripheral ganglia near or in the heart

- axons: project to targets

- targets: SA node, internodal tracts, atrial muscle, AV node

- ventricles receive virtually no input from parasympathetic nerves

- neurotransmitter: acetylcholine

- receptor: muscarinic (M2)

- function: slows heart rate, conduction velocity

- cardio-inhibitory center (CIC): cell bodies of the preganglionic parasympathetic neurons

- diffuse bilateral center in the medulla

- tends to dominate at rest

- sympathetic

- preganglionic

- cell bodies: lateral horns of the spinal cord, T1 to T6

- axons: synapse in paravertebral chains

- neurotransmitter: acetylcholine

- postganglionic

- cell bodies: paravertebral chains

- axons: project to the heart via the superior, middle, and inferior nerves

- targets: all structures of the heart, including the ventricles

- neurotransmitter: norepinephrine

- receptor: adrenergic (β1)

- cardio-accelerator center (CAC): neuronal control of preganglionic sympathetics

- diffuse bilateral center in the brainstem

- synapse on lateral horn cells

functions of the heart regulated by autonomic nerves

- heart rate

- chronotropism: change in the rate of the heart beat

- positive chronotropism: increased heart rate

- negative chronotropism: decreased heart rate

- mechanism: changes in the rate of diastolic depolarization

- conduction of excitation

- dromotropism: induced changes in action potential conduction velocity

- AV node: functionally most important in controlling conduction velocity

- anything arising from SA node must traverse AV node to control ventricular excitation

- mechanism: changes in the intensity of the depolarizing current (Na+ or Ca2+)

- alters upstroke velocity of the action potential

- alters overshoot of the action potential

- contraction force and velocity

- inotropism: induced changes in myocardial contractile force, resulting in altered stroke volume

- positive inotropy: increase in developed force (or pressure)

- negative inotropy: decrease in developed force (or pressure)

- mechanisms

- change in magnitude of slow Ca2+ current

- modulation of Ca2+ release

- modulation of Ca2+ binding

- modulation of metabolic energy balance

- excitability or irritability

- excitability

- laboratory definition: tendency of the tissue to respond to a stimulus

- clinical definition: tendency of the heart to develop arrythmias

- as such, excitability is often also referred to as irritability

cardiac actions of the parasympathetic nerves

- decreases heart rate

- mechanism: influences diastolic depolarization (phase 0) ionic currents via ACh binding to M2 receptors

- increases membrane K+ conductance (gK), slowing rate of membrane depolarization

- reduces intensity of Na+ current through If channels

- blocks T-type Ca2+ current

- selective effect: targets only atrial walls, AV node

- decreases conduction velocity through AV node

- mechanism

- ACh reduces magnitude of L-type Ca2+ current

- causes decrease in upstroke velocity, overshoot, and thus conduction velocity

- effect: longer time for excitation to traverse AV node, longer P-R interval

- note: can sometimes increase conduction velocity along atrial muscle cells

- ACh-induced increase in membrane gK hyperpolarizes resting membrane potential (-90 to -100 mV)

- increases number of available fast Na+ channels, ultimately increasing conduction velocity

- decreases contractility of atrial muscle cells

- mechanism: decreased amplitude of L-type Ca2+ current (required to initiate Ca2+ release from SR)

- result: negative inotropy, decrease in atrial kick

- selective effects: parasympathetics target atrial walls and AV node, but do not work well in ventricles

- significantly fewer muscarinic receptors in Purkinje cells, ventricular muscle

- significantly less innervation of ventricular cells

cardiac actions of sympathetic nerves

- increases heart rate

- mechanism: influences ionic currents via norepinephrine binding to β1 receptors (PKA-mediated)

- accelerated rate of gK decrease

- increased inward movement of Na+ through If channels

- increased inward Ca2+ channel current through T-type channels

- broad effect: all latent pacemakers receive sympathetic innervation, all have β1 receptors

- increases conduction velocity through AV node

- nodal cells: increase in rate of rise and amount of L-type Ca2+ current

- causes significant increase in AV upstroke, overshoot, and thus conduction velocity

- effect: shorter time for excitation to traverse AV node, shorter P-R interval

- non-nodal cells: mechanism is unclear; may involve hyperpolarization of the threshold potential

- increases contractility of muscle cells

- positive inotropy

- mechanism: upregulation of adenylate cyclase, consequent increase in active PKA

- increase in magnitude, duration of slow L-type Ca2+ current; consequent increase in [Ca2+]i

- increased Ca2+ release from intracellular stores

- increased rate of cross-bridge cycling

- result: increase in atrial kick, and consequent increase in ventricular stroke volume

- increased rate of relaxation

- mechanism: myofibrillar protein phosphorylations (primarily TnI subunit)

- increased rate of cross bridge cycling (length of rate-limiting steps is reduced)

- increased Ca2+ reuptake by SR

- mediated by phosphorylation of phospholamban

- phospholamban: accessory protein to the SR Ca2+ pump

- result: increase in rate of relaxation

- net effect: increase amplitude, reduce duration of cardiac twitch

- increases excitability of cardiac tissue

- mechanism: unclear

- threshold closer to max diastolic potential

- automaticity of latent pacemakers is increased

- problem: hearts subjected to excessive sympathetic innervation tend to develop arrythmias

levels of cardiac autonomic nerve activity in normal humans at rest

- SA node automaticity: 120 beats/minute in absence of outside influence

- normal resting heart rate: 60-80 beats/min

- implication: at rest, heart has a generally parasympathetic tone

TABLE: Summary of Cardiac Effects of Autonomic Nervous System

parasympathetics

sympathetics

target

atria

atria, ventricles

mechanism

anti-β effects (↓ PKA)

↑ PKA (protein, channel phosphorylation)

chronotropy

(heart rate)

decrease (negative chronotropy)

↑ gK

↑ If

↑ ICa

increase (positive chronotropy)

accelerated reduction in gK

↓ If

↓ ICa

dromotropy

(conduction velocity)

decrease (through AV node)

↓ L-type ICa

increase (through AV node)

↑ L-type ICa

inotropy

(force, speed)

decrease (negative inotropy)

↓ L-type ICa

increase (positive inotropy)

↑ L-type ICa

↑ SR Ca2+ release

↑ SR Ca2+ reuptake speed

↑ cross bridge kinetics

excitability

(irritability)

decrease

↑ gK

increase

mechanism unclear: threshold closer to max diastolic potential, automaticity of latent pacemakers is increased

Hemodynamics

- hemodynamics: study of the physical factors that produce and affect blood flow

- based on idealized laws developed to quantify flow of liquid through tubes

- quantitatively apply only when liquid is Newtonian in nature (ideal)

- nevertheless, can aid in illustrating general properties on which blood flow is based

physical properties of flow

- pressure

- pressure is form of energy that causes liquid to flow

- flow occurs when pressure exceeds resistance

- resistance is presented by the tube, inertia of the liquid

- pressure can be converted to other forms of energy

- kinetic energy: energy of flow

- heat: byproduct of overcoming resistance to flow

- pressure energy in a tube is a sum of lateral and end pressure

- energy:

- lateral pressure: pressure exerted on the walls of the container (potential energy)

- end pressure: pressure exerted by energy of flow (kinetic energy)

- note that measurement of flow is measurement of total energy, not just of kinetic energy

- resistance

- determinants

- length of tube: resistance is proportional to tube length

- radius of tube: resistance is inversely exponentially proportional to tube radius

- fluid viscosity: resistance is inversely proportional to viscosity

- measurement

- resistance:

- L: tube length

- r: tube radius

- η: fluid viscosity

- even a small change in radius will profoundly alter resistance (R is proportional to 1/r4)

- flow

- Poiseluille’s law:

- Q: flow

- ΔP: pressure gradient

- implications

- flow is directly proportional to size of the pressure gradient and radius of the vessel

- flow is inversely proportional to length of the vessel and viscosity of the fluid

practical applications

- driving force of flow: systemic arterial blood pressure

- physical properties of the vasculature

- parallel arrangement: vascular beds of systemic circulation are arranged in parallel

- pressure in arteries supplying each bed is nearly the same

- same driving force for flow exists at entrance to each bed

- consequently, increased pressure is not efficient in selective flow

- resistance: flow through each vascular bed is primarily determined by net radius of the vessels

- resistance to flow depends on the radius of the vessels

- radius of vessels is primarily determined by contraction/relaxation of circular smooth muscles

- several mechanisms exist to control this

- arterioles: narrow, high resistance vessels located at the end of the arterial tree

- divides systemic circuit into a high pressure region and a low pressure region

- effect: decreases velocity of flow

- increases upstream pressure, decreases downstream pressure

- end result is to increase pressure gradient across the constricted region

vascular resistance

- resistance:

- ΔP: mean arterial blood pressure (MABP) - RA pressure

- RA pressure is generally close to zero

- ΔP is thus approximated as MABP

- Q: flow

- total peripheral resistance (TPR)

- theory

- ΔP: approximated as MABP, since RA pressure is typically close to 0

- TPR is the primary determinant of MABP

- Q can be thought of as cardiac output (CO)

- equation:

- MABP: mean arterial blood pressure

- CO: cardiac output

- TPR is less than the largest of the individual resistances

- consequence of parallel arrangement of vascular beds

- resistance in series:

velocity of flow

- velocity: length of a column of blood passing a point in the circuit per unit time

- velocity is inversely proportional to cross-sectional area of the vessel

- thus as vessel gets bigger, velocity (and kinetic energy) decrease

- implications

- velocity is rapid in arteries and veins, but slow in capillaries

- allows time for exchange of materials across the capillary wall

Bernoulli’s principle

- Bernoulli’s principle: total energy (E = PE + KE) at any point in a system must remain constant

- sum of lateral pressure (PE) and end pressure (KE) must remain constant

- implication: areas of decreased flow receive additional lateral pressure

- arterial aneurism

- bulging of vessel causes reduction in flow and increase in lateral pressure

- as such, aneurisms tend to be unstable and progressive

patterns of blood flow within vessels

- patterns of flow

- streamlined (laminar) flow

- liquid is layered as it flows, and flow occurs without intermixing of the fluid

- each layer (lamella) moves at a different velocity, with greatest velocity at the center

- pattern: ordered, structured, efficient, and quiet

- turbulent flow

- liquid rolls, mixes together as it flows

- pattern: less efficient, requires higher pressure, and is audible

- describing turbulence

- Reynold’s number:

- v: velocity of flow

- D: diameter of tube

- d: density of liquid

- η: viscosity of liquid

- as Re increases, likelihood of turbulence in flow also increases

- with all else constant, faster flow à increased turbulence

- clinical applications

- abnormal constrictions of dilations of vessels can cause audible downstream turbulence (brutis)

- anemia reduces viscosity of blood, which can result in turbulence

- sphygmomanometry relies on turbulent flow sounds in vessels constricted with a pressure cuff

heart sounds and murmurs

- listening to heart sounds

- stethoscope: that amplifying thingy

- ascultation: the technique of listening for sounds anywhere in circulation

- normal heart sound

- S1: AV valve closure onset of systole “lub”

- S2: SL valve closure onset of diastole “dub”

- heart murmur

- terms

- valvular insufficiency: condition in which blood is able to regurgitate backward through a closed valve

- valvular stenosis: condition in which a valve does not open sufficiently during movement of blood

- findings

- systolic murmur from AV valve: insufficiency (systole: AV valve closes)

- systolic murmur from SL valve: stenosis

- diastolic murmur from AV valve: stenosis (diastole: SL valve closes)

- diastolic murmur from SL valve: insufficiency

LaPlace’s law for wall tension in blood vessels

- LaPlace’s law: T = P · r

- P: intraluminal pressure

- r: radius of vessel

- implications

- pressure is smaller, and radius is far smaller in capillaries than arteries

- as such, the tension is significantly lower, allowing capillary walls to be much thinner

Regulation of Contraction of Vascular Smooth Muscle

- functions of contraction in the cardiovascular system

- reduce the radius of the vessel

- contraction increases resistance to blood flow through the vessel

- arterioles: most important, as they determine TPR

- reduce the capacity of the vessel

- contraction decreases diameter of the lumen, decreasing vessel capacity

- systemic veins: most important, as a reduction in venous capacity causes more blood return to the heart

- increases cardiac output

- increases arterial blood pressure

- reduce the distensibility of the vessel

vascular resistance

- peripheral resistance

- resistance to flow is almost entirely within peripheral vessels, as opposed to the heart

- calculation: depends on pressure gradient (ΔP), flow (Q) across a bed

- total peripheral resistance

- systemic vascular beds arranged in parallel

- inverse of TPR is the sum of the inverses of all vascular beds

- consequence: TPR is less than the resistance of any single vascular bed

- arteriolar smooth muscle: major determinant of TPR

- arterioles

- located at entrance to each capillary

- ideally positioned to control capillary blood flow

- capillaries

- precapillary sphincter: smooth muscle located at the entrance of the capillary

- determines actual blood flow through individual capillary

terms applied to change in vessel radius

- vasoconstriction: decrease in radius of a blood vessel

- types

- passive: recoil due to fall in intraluminal pressure

- active: contraction of smooth muscle in the vessel wall

- contractile factors

- activity in sympathetic adrenergic nerves (primary importance in controlling TPR, capacity, and MABP)

- hormones and neurotransmitters

- exogenous therapeutic agents

- vasodilation: increase in radius of a blood vessel

- types

- passive: stretch due to increase in intraluminal pressure

- active: relaxation of smooth muscle in the vessel wall

- contractile factors

- inhibition of sympathetic adrenergic nerves (important for controlling TPR and MABP)

- therapeutic agents

- local chemical changes produced by tissue metabolism or vascular endothelium

innervation of vascular smooth muscle

- distribution

- all vascular beds receive sympathetic adrenergic innervation

- anatomy

- preganglionic sympathetic: lateral horn of the spinal cord

- postganglionic sympathetic: paravertebral chain

- density of innervation is significantly reduced in:

- brain

- coronary arteries

- pulmonary circulation (determined primarily by local [O2], [CO2])

- sympathetic vasoconstriction: α1 receptors

- vasomotor center: bilateral controller of sympathetic innervation

- located in the medulla

- controls preganglionic sympathetic constrictor neurons in thoracic, lumbar segments (T1-L2)

- sympathetic innervation of smooth muscle contraction

- neurotransmitter: norepinephrine

- receptors: α1 receptors on vascular smooth muscle cells

- distribution

- skin

- skeletal muscle

- bone

- abdominal and pelvic regions (splanchnic vascular beds)

- effect: actively controls TPR and venous capacity

- a tonically-active (3-5 Hz) single set of nerves regulates TPR over a broad range

- increase or decrease of this firing frequency causes resultant changes in TPR, MABP

- sympathetic vasodilation: β2 receptors

- sympathetic innervation of smooth muscle contraction

- neurotransmitter: norepinephrine

- receptors: β2 receptors on vascular smooth muscle cells

- distribution: skeletal muscle beds

- effect: offsets vasoconstriction due to sympathetic adrenergic neurons, to some degree

- beneficial effects in facilitating blood flow to skeletal muscle during exercise

- overall, local [metabolite] dominates, and β2 receptors don’t play that much of a role

regulation of vascular smooth muscle contraction

- local metabolites

- vasodilatory factors

- decreased PO2

- increased PCO2

- decreased pH

- increased adenosine

- increased extracellular [K+]

- increased osmolarity

- increased temperature

- advantage: localized response

- metabolism stimulates changes, leading to increased blood flow on an “as needed” basis

- highly efficient at increasing blood flow to a specific organ or vascular bed

- endothelial cells

- endothelium: single layer of cells lining or comprising the luminal wall of blood vessels

- functions

- formation of a diffusion barrier between circulation and surrounding fluid compartments

- formation of a smooth surface for interaction with blood cells

- modulation and synthesis of numerous blood-borne substances

- modulation or control of contractile state of smooth muscle

- examples

- nitric oxide (NO)

- synthesis and release induced by shear stress across the endothelial surface (mechanism unknown)

- effect: localized vasodilation (reduces velocity of flow, prevents further injury)

- acetylcholine (ACh)

- binds muscarinic receptors on endothelial cells, inducing synthesis and release of NO

- effect: localized vasodilation

- function: unknown

- ACh does not circulate in the blood

- few vessels receive cholinergic innervation

- use as a diagnostic tool

- normal tissues: vasodilate in response to ACh due to interaction with endothelium

- damaged tissues: constrict in response to ACh due to loss of endothelium, interaction with smooth muscle

- other substances

- vasodilation: response to histamine, bradykinin

- vasocontstriction: response to arachidonic acid, stretch

- monoamine oxidase: endothelial enzyme that inactivates norepinephrine and serotonin

- platelet aggregation, blood coagulation: induced by numerous factors released by the endothelium

- myogenic response

- stretch reflex

- effect: contraction in response to simple stretch

- function: allows greater precision in control of flow to a vascular bed

- increasing stimuli for vasodilation (metabolites, pressure) are counteracted by stretch reflex

- at high stimulus intensity, stretch reflex becomes less effective

- mechanism: stretch activation of membrane Ca2+ channels

- inward current depolarizes cell, induces contraction

- self-limiting: stretch-induced contraction reduces cell length, thereby reducing conductance of Ca2+ channels

- atrial natriuretic factor (ANF)

- released by atrial, ventricular myocytes in response to increased EDV

- targets: kidney, adrenal glands, certain regions of the brain

- effects: reduce blood pressure

- diuresis (increased urine output)

- natriuresis (increased Na+ content of the urine)

- vasodilation

- renin-angiotensin-aldosterone system

- pathway: formation of angiotensin II

- renin is secreted in response to:

- reflex activation of sympathetic outflow to the justaglomerular apparatus

- direct release from renal afferent arterioles in response to low blood pressure

- renin catalyzes cleavage of circulating angiotensinogen into angiotensin I

- angiotensin-converting enzyme (ACE) catalyzes cleavage of angiotensin I into angiotensin II

- ACE found primarily in endothelial surfaces of lung vasculature

- angiotensin II: effects to increase blood pressure

- vasoconstrictor

- stimulates Na+ absorption, thereby increasing water retention

- stimulates aldosterone release from the adrenal cortex, which increases Na+ reabsorption by the kidney

- stimulates hypothalamic centers to increase thirst

autoregulation

- autoregulation: maintenance of constant flow regardless of perfusion pressure

- with elevated pressure, flow decreases back to normal due to vasoconstriction

- with reduced pressure, flow increases to normal due to vasodilation

- mechanisms

- myogenic response: appropriate activation of the stretch reflex

- increased local metabolites: appropriate synthesis of metabolites to restore flow rate

reactive hyperemia

- reactive hyperemia: greater than normal volume of blood flow following a period of zero blood flow

- mechanism: abundance of vasodilating agents

- during zero flow, changes in pH, PO2, PCO2, and other metabolites favor vasodilation

- upon restoration, flow is initially greater than normal but recovers once metabolites are cleared

- longer occlusion à longer hyperemia

Neural Regulation of Mean Arterial Blood Pressure

determinants of MABP

- amount of blood in arteries: depends on amount entering vs. amount leaving arterial tree

- CO: cardiac output amount of blood entering arterial tree

- TPR: total peripheral resistance determines blood leaving arterial tree

- cardiac output: depends on heart rate and stroke volume

- HR: heart rate

- SV: stroke volume

- influences on variables in maintaining constant MABP

- cardio-inhibitory center (CI): heart rate and stroke volume (CO)

- cardio-accelerator center (CA): heart rate and stroke volume (CO)

- vasoconstrictor center (VC): total peripheral resistance (TPR)

mechanisms influencing activity of CI, CA, and VC centers

- neural influences from higher centers in the brain

- centers in the cortex, hypothalamus:

- alter output of impulses from CI, CA, and VC centers

- emotional states

- activate centers in the brainstem (CI, CA, VC)

- effect the release of epinephrine from the adrenal glands

- body temperature control centers in the hypothalamus

- influence pool of VC neurons responsible for control of blood flow to the skin

- local chemical effects within the brain

- effects of PCO2

- VC center: highly sensitive to cerebral PCO2 (interstitial fluid)

- 45 mmHg: normal PCO2

- increased PCO2: increase in VC activity effecting vasoconstriction, increase in TPR

- decreased PCO2: decrease in VC activity allowing vasodilation, decrease in TPR

- mechanism

- direct effects on centers due to changes in [H+]

- note: vasoconstriction does not affect brain and heart due to reduced sympathetic innervation

- Cushing’s phenomenon: compensatory increase in MABP in response to increase in brain CO2

- during trauma, blood flow to the brain decreases, CO2 is not cleared as fast, and PCO2 increases

- VC center stimulated, causing systemic vasoconstriction and restoring blood flow to the brain

- note: vascular beds of brain, heart do NOT participate in generalized vasoconstriction (less innervation)

- voluntary hyperventilation

- causes decreased PCO2, effecting a reduced tone in the VC center

- TPR falls, venous capacity increases, and MABP falls

- if MABP falls too much, there is inadequate cerebral blood flow and unconsciousness may result

- CI, CA centers

- increased cerebral PCO2: stimulates both the CA and CI centers

- CI typically dominates, and counterintuitively, HR decreases

- mechanism: direct effects on centers due to changes in [H+]

- effects of PO2

- within brain: small ΔPO2 has little effect; large reductions can cause depressed neural activity

- outside brain: other mechanisms exist by which altered PO2 can stimulate CV system

reflex effects from peripheral neural receptors: arterial baroreceptors

- location and innervation

- carotid sinus baroreceptors: glossopharyngeal (CN IX) nerve

- aortic arch baroreceptors: vagus (CN X) nerve

- mechanism: stretch receptors embedded in vessel walls

- magnitude: effects depend on frequency, duration, and number of active receptors

- tonic activation: some activated during each beat of the cardiac cycle

- frequency: increased discharge, duration with increasing MABP

- recruitment: increased numbers activated with increased MABP

- tonic effects of baroreceptors

- stimulate the CI center

- CI center is tonically active due to constant stimulation by baroreceptors

- inherent SA discharge: 120 beats/min

- normal resting discharge: 60-80 beats/min

- modifying activation

- increasing MABP: increased stimulation, decreased heart rate

- decreasing MABP: decreased stimulation, increased heart rate

- inhibit CA center

- CA center is minimally active due to tonic inhibition by baroreceptors

- modifying activation

- increasing MABP: increased inhibition, decreased heart rate/contraction force

- decreasing MABP: decreased inhibition, increased heart rate/contraction force

- inhibit VC center

- VC center is tonically active, but less than normal due to constant inhibition by baroreceptors

- inherent discharge (normal PCO2) frequency = 10 Hz

- resting discharge rate: frequency = 3-5 Hz

- modifying activation

- increasing MABP: increased inhibition, decreased sympathetic vasoconstriction (vasodilation)

- decreasing MABP: decreased inhibition, increased sympathetic vasoconstriction

TABLE: Summary of Tonic Effects of Baroreceptors

activity

method

action

effect

stimulate CIC

↓ HR

↓ CO

↓ MABP

inhibit CAC

↓ HR, SV

↓ CO

inhibit VC

vasodilation

↓ TPR, ↓ CO

- “buffer” reflexes

- baroreceptors cannot completely prevent any change in arterial pressure

- instead, they provide fast, effective, moment-to-moment regulation of MABP

- with prolonged abnormality, reflexes do adapt to a new standard

- hemorrage: loss of blood directly from the cardiovascular system

- hemorrhage causes a fall in arterial pressure:

- direct loss from the arterial tree

- reduction in blood return to the heart

- response: decreased activity of baroreceptors leading to increased MABP

- CI no longer stimulated, resulting in increased heart rate

- CA no longer inhibited, resulting in increased heart rate and force of contraction

- VC no longer inhibited, resulting in constricted vessels and increased TPR

- function

- mild hemorrhage (<30%>

- severe hemorrhage: prolongs survival time of the organism

- value

- heart is able to pump all blood it receives

- blood is diverted to the brain and heart, which most need blood

- injection of a direct vasoconstrictor drug

- injection of a vasoconstrictor causes constriction of vessels leading to increased TPR, increased MABP

- response: activation of baroreceptors

- CI center activated above normal, slowing heart rate

- CA center further inhibited, reducing heart rate and contraction strength

- VC center further inhibited, reducing constriction and lowering CO

- effect: pharmacologic increase in MABP is moderated

effects on MABP due to stimulation of peripheral chemoreceptors

- structures

- carotid bodies: bilateral structures located at the bifurcation of the common carotid arteries

- aortic bodies: located near the aortic arch

- innervation: glossopharyngeal, vagus nerve

- synapse on CV control centers (especially VC center)

- synapse on respiratory centers

- stimulation

- minimally active at rest

- respond to changes in arterial blood concentrations

- decreases in PO2

- increases in PCO2

- decreases in pH

- effect: increases blood flow to vital organs (brain and heart) when PO2 is low

- stimulates VC center: ↑ TPR gives ↑ MABP

- stimulates CA center: ↑ CO gives ↑ MABP

cardio-pulmonary reflexes

- overview

- receptors: imbedded in walls of the heart, large thoracic vessels, and lungs

- effect: modify vasomotor tone, heart rate, breathing

- innervation: afferents project with the vagus nerve

- ventricular receptors

- activation: greater than normal increases in diastolic or systolic pressure

- effect: strong depressor

- Bezold-Jarisch reflex

- severe depressor response caused by ventricular distension

- results in hypotension, bradycardia, and even fainting (may explain fright response in some)

- hypertension can make a person more susceptible to evoking the reflex

- somatic afferent reflexes

- types of responses

- pressor response: increases in blood pressure and heart rate

- depressor response: decreases in blood pressure and heart rate

- stimulation of somatic afferents generally cause a pressor response

- example: increased BP, heart rate in response to pain

- some have depressor responses, though the mechanism and function is unknown

summary of regulation

- baroreceptor reflex: dominates control

- (+) CI: ↓ HR directly reduces HR (M-ACh)

- (–) VC: ↓ TPR less arterial, venous vasoconstriction 1 adrenergic)

- (–) CA: ↓ HR, contractility less SA node, myocyte stimulation 1 adrenergic)

TABLE: Receptors in the Cardiovascular System

location

action

M-ACh (M1)

SA node

↓ heart rate

N-ACh (ganglionic)

autonomic ganglia

postganglionic EPSP

α1 adrenergic

arterioles, veins

vasoconstriction

β1 adrenergic

SA node

cardiac muscle cells

↑ heart rate

↑ contractile strength

β2 adrenergic

some arterioles

vasodilation

guanylyl cyclase

arterioles

vasodilation

- receptor affinities

- norepinephrine: strong with α1, β1 (α and β)

- epinephrine: strong with β2, β1 (primarily β)

- mathematical relationships

- influences on stroke volume

- ↑ preload (HR): (–) effect

- ↑ afterload (MABP) (–) effect

- ↑ contractility (β1 stimulation): (+) effect

- reflex can be overridden

- emotions

- Cushing’s phenomenon (central chemoreceptors)

- peripheral chemoreceptors

- cardiopulmonary reflexes

- volume receptors (JGA in the kidney)

- local metabolites

Arterial Blood Pressure and Pulse

measurement of arterial blood pressure

- sphygmomanometry: most common method of measuring arterial blood pressure

- cuff pressure is increased above arterial blood pressure, occluding the pulse

- systolic: spurting of blood into arteries, causing audible turbulence and palpable radial pulses

- diastolic: reestablishment of laminar flow, causing loss of turbulence

- Korotkoff’s sounds: sound of turbulent flow heard during measurement of blood pressure

- average values

- young adult males: 120/80

- young adult females: 110/70

arterial pressure wave

- arterial pulse wave: propagating expansion of the arterial walls

- initiated at the root of the aorta upon ejection of a stroke of blood

- rate: 5-15 m/s (faster than flow velocity of 0.3-0.5 m/s)

- length: 5-7 m (longer than the longest single path in the cardiovascular system)

- can be felt upon palpation of an artery

- partially reflected by arterial bifurcations, small caliber vessels deep in arterial tree

- normally, only arrive back at root after aortic valve has closed

- with stiffened arterial tree, velocity is increased

- can arrive back at root while aortic valve is still open

- increases peak systolic pressure, pulse pressure

Capillary Function

anatomical considerations

- size

- internal diameter: 8 μm or less

- length: 0.5 to 1.0 mm

- density: correlates with metabolic activity of the tissue

- heart muscle: 2500-4000 capillaries / mm3

- skin: 100 capillaries / mm3

- pores: slit-like openings in capillary walls

- found at junctions of endothelial cells

- vary in size and number in different capillaries

- large: liver and marrow (allow passage of proteins in liver, RBCs in marrow)

- small: small (reduce, prevent loss of proteins and cellular elements from the IV space)

- occupy only about 0.02% of capillary surface area

movement of substances across the capillary wall

- diffusion: passive movement across a concentration gradient

- water soluble, fat insoluble: limited to diffusion through pores (inorganic ions, water, glucose, urea)

- fat soluble: can pass across the wall at any point (O2, CO2)

- responsible for vast majority of substance transfer

- filtration/reabsorption: movement due to pressure differences between lumen, interstitial fluid space

- fluid (filtrate) moves through the pores, carrying all solutes small enough to pass through

- mechanism

- pressure components

- hydrostatic pressure: capillary blood pressure (tissue hydrostatic pressure assumed to be zero)

- causes filtration, opposes reabsorption

- declines steadily along the length of the capillary (~32 to ~15 mmHg)

- oncotic pressure: osmotic pressure difference between plasma, interstitial fluid

- develops due to a difference in protein concentration between the fluids

- ~25 mmHg

- capillary exchange

- filtration: initial segment of the capillary (hydrostatic pressure exceeds oncotic, tissue pressures)

- reabsorption: final segment of the capillary (oncotic, tissue pressures exceed hydrostatic pressure)

- responsible for only a small portion of substance transfer, relative to diffusion

- causes of edema

- abnormal, sustained rise in systemic pressure

- reabsorption is reduced, resulting in edema if this exceeds the capacity of the lymphatics to drain fluid

- secondary to several cardiovascular diseases

- decrease in [plasma protein]

- oncotic pressure falls, causing a decrease in the force opposing filtration and promoting reabsorption

- secondary to starvation, kidney diseases, or liver diseases

- abnormally high permeability of the capillary wall

- filtration is increased

- secondary to chemicals such as histamine, bacterial toxins

- obstruction of lymphatic vessels

- drainage of the interstitial fluid space is decreased

- secondary to tumors, surgical complications, or parasites / infections

control of capillary blood flow

- control point: pre-capillary sphincter

- contraction: narrows opening, restricting passage of blood

- relaxation: expands opening, allowing greater passage of blood

- control primarily involves local metabolites rather than nerve activity

- vasomotion: rhythmic contraction and relaxation of pre-capillary sphincters

- spontaneously occurs even during tissue rest, constant metabolism

- due to highly localized changes in metabolite concentration

The Systemic Venous System

effects of gravity on distribution of venous blood

- gravity works against adequate circulation

- standing human: 70% of systemic circulation below the level of the heart

- gravity increases pressure of blood in direct proportion to distance below the heart

- gravity decreases pressure of blood in direct proportion to distance above the heart

- in venous return, gravity is potentially detrimental

- causes distension of systemic veins

- can allow pooling of blood, reducing cardiac output

- pressure alone can drive venous return in the recumbent individual

- pressure at entrance to veins: 15 mmHg

- pressure near RA: ~0 mmHg

- accessory mechanisms are needed for venous return in the upright individual

accessory factors promoting venous return in upright humans

- neurally-induced contraction of venous smooth muscle

- drive: sympathetic adrenergic vasoconstrictor neurons

- venous capacity is kept from increasing, blood does not accumulate

- pumping action of skeletal muscles

- valve: flaps of endothelial tissue that prevent backflow in the veins

- localized primarily to the limbs

- pumping of skeletal muscles causes unidirectional movement back towards the heart

- respiratory pump

- inspiration: drives return towards the heart

- enlarged thorax: fall in intrathoracic pressure

- large veins near the heart are thin enough that this reduces IV pressure

- creates a pressure gradient that favors flow of blood from extrathoracic regions

- descent of the diaphragm: rise in abdominal pressure, further increasing return

- expiration: backflow prevented by valves

venous (jugular) pulse

- external jugular veins: exhibit pulsations in the neck during each beat

- result from transient RA pressure increases

- contraction: a wave

- ventricular filling/release (bulges affect atria): c wave, x descent

- atrial filling/release: v wave, y descent

- especially evident when the head is in a lower position than the feet

- certain valvular defects, cardiac arrhythmias can predictably alter jugular pulse

The Pulmonary Circulation

hemodynamics of the pulmonary circulation

- pressures

- right ventricle systolic: 22-25 mmHg diastolic: 0-5 mmHg

- pulmonary artery: systolic: 22-25 mmHg diastolic: 8-10 mmHg

- pulmonary capillary: 6-8 mmHg

- left atrium: 5-7 mmHg

- flow

- blood flow is necessarily identical to the amount flowing through the systemic system

- driven by a small pressure gradient

- pulmonary arteries are shorter, wider than systemic counterparts

- resistance of pulmonary system is thus quite low

- high distensibility of the pulmonary system: illustrations

- cardiac output

- resting: 5 L/min

- exercising: 25 L/min

- accompanied primarily by enlarging of pulmonary vessels, rather than increase in pulmonary pressure

- surgical removal of one lung

- results in diversion of entire normal cardiac output to one lung

- pulmonary pressure still does not increase, again due to distensibility of the vessels

pulmonary artery (capillary) wedge pressure

- clinically useful

- filling pressure for the left ventricle

- determines left ventricular cell lengths at end-diastole

- difficult measurement to make

- pass a catheter into R side of the heart via systemic veins

- pass it into pulmonary circulation until it wedges in a small vessel

- recorded pressure is close to that of the L atrium

distribution of blood within the lungs

- highly susceptible to gravity

- pressures within circulation are low

- vessels are distensible

- pressure varies with position relative to heart

- apex: above the heart; relatively lower pressure

- base: below the heart; relatively higher pressure

- amount of blood perfusing these regions differs in a resting person, can vary more with upright individual

filtration/reabsorption in the lungs

- normal individuals: alveoli are “dry”

- pulmonary capillary pressure less than plasma oncotic pressure

- interstitial fluid volume remains normal

- pulmonary edema: accumulation of fluid in the interstitium and alveolar space

- commonly caused by left heart failure, which causes blood pooling in the pulmonary circulation

- can also be caused by mitral, aortic valve stenosis

blood volume in lungs

- pulmonary blood volume: 15-20% of total blood volume

- varies slightly with changes in body position

- largest when an individual is recumbent

- smallest when upright

- pulmonary edema manifests more when an individual is recumbent

regulation of pulmonary blood flow

- sympathetic innervation

- sympathetic vasoconstriction

- function: not well understood; may not be important

- local regulatory mechanisms

- hypoxia: vasoconstriction (opposite that in systemic circulation)

- must develop in the air passages, not in the blood

- acts to reduce flow of blood through regions in which O2 supply is inadequate for exchange

- high O2: vasodilation

- can, to some degree, act as a reservoir for blood

- can accept or give up significant quantities of blood

- can aid cardiac filling for a few beats or help maintain appropriate capacity-volume relationship